CBSE-2023 Syllabus for Biotechnology Classes XII
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Theory 3 Hours and 70 Marks and 180 Peroids
Unit V: Protein and Gene Manipulation Marks 40 (100 Periods)
Chapter I: Recombinant DNA Technology 15 Marks (40 Periods)
Introduction
- Tool of rDNATechnology
- Making rDNA
- Introduction of Recombinant DNA into Host Cells
- Identification of Recombinants
- Polymerase Chain Reaction (PCR)
- Hybridization Techniques
- DNA Library
- DNA Sequencing
- Site-directed Mutagenesis
Chapter II: Protein Structure and Engineering 15 Marks (35 Periods)
Introduction to the World of Proteins
- 3 − D Shape of Proteins
- Structure-Function Relationship in Proteins
- Purification of Proteins
- Characterization of Proteins
- Protein Based Products
- Designing Proteins (Protein Engineering)
Chapter III: Genomics and Bioinformatics 10 Marks (25 Periods)
Introduction
- Genome Sequencing Projects
- Gene Prediction and Counting
- Genome Similarity, SNPs and Comparative Genomics
- Functional Genomics
- Proteomics
- History of Bioinformatics
- Sequences and Nomenclature
- Information Sources
- Analysis using Bioinformatics Tools
Unit VI: Cell Culture and Genetic Manipulation 30 Marks (80 Periods)
Chapter I: Microbial Culture and Applications 10 Marks (26 Periods)
Introduction
- Microbial Culture Techniques
- Measurement and Kinetics of Microbial Growth
- Scale up of Microbial Process
- Isolation of Microbial Products
- Strain isolation and Improvement
- Applications of Microbial Culture Technology
- Biosafety Issues in Microbial Technology
Chapter II: Plant Cell Culture and Applications 10 Marks (27 Periods)
Introduction
- Cell and Tissue Culture Techniques
- Applications of Cell and Tissue Culture
- Gene Transfer Methods in Plants
- Transgenic Plants with Beneficial Traits
- Biosafety in Plant Genetic Engineering
Chapter III: Animal Cell Culture and Applications 10 Marks (27 Periods)
Introduction
- Animal Cell Culture Techniques
- Characterisation of Cell Lines
- Methods of Gene Delivery into Cells
- Scale-up of Animal Culture Process
- Applications of Animal Cell Culture
- Stem Cell Technology
- Tissue Engineering
Practicals
Note: Every student will be required to do the following experiments during the academic session
List of Experiments
- Isolation of bacterial plasmid DNA and its detection by gel electrophoresis
- Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
- Bacterial transformation using any plasmid
- Data retreival and data base search using internet site NCBI
- Download a DNA and protein sequence from internet, analyse it and comment on it.
- Cell viability assay
- Determination of blood groups
- Estimation of DNA
- Ion-exchange chromatography for proteins.
- Reading of DNA sequencing gel to arrive at the sequence
- Estimation of blood glucose by enzymatic method (GOD/POD)
- Project work
Scheme of Evaluation Time is 3 Hours and 30 Marks
The scheme of evaluation at the end of the session will be as under:
- Two experiments: 6 + 6 (only one computer based practical)
- Practical record: 04
- Viva on Practicals: 04
- Project work:
- Write up: 05
- Viva on project: 05
- Total 30
Recommended Books
- A Text Book of Biotechnology-Class XI: Published by CBSE, New Delhi
- A Laboratory Manual of Biotechnology-Class XI: Published by CBSE, New Delhi
- A Text Book of Biotechnology-Class XII: Published by CBSE, New Delhi
- A Laboratory Manual of Biotechnology-Class XII: Published by CBSE, New Delhi