Biotechnology Question Bank, General Biotechnology, Traditional Biotechnology and Modern Biotechnology

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Questions of General Biotechnology

Q1. What is biotechnology? What are the achievements of biotechnology?

Q2. Write a short note on the global impact of biotechnology.

Q3. Write a short note on History of biotechnology highlighting the contributions of various scientists.

Q4. What is the difference between the traditional biotechnology and modern biotechnology?

Q5. Write the full forms of the following terms: NBTB, DBT, OECD ICGEB.

Questions on Basic Concepts

Q1. What is PCR technology? What are its applications?

Q2. What is restriction and modification system?

Q3. Give a detailed account of Restriction endonucleases.

Q4. What are plasmids? How do they work as cloning vector?

Q5. What is a cloning vector? Why are they necessary?

Q6. Write short notes on: a) YAC vectors, b) pBR322, c) BAC vector, d) Cosmid vectors.

Q7. What is site directed mutagenesis?

Q8. What is genomics? Give a brief account of genome sequencing project.

Q9. What is the ‘microarray technique’ used to differentiate a normal cell from a diseased cell?

Q10. What do you mean by ‘complete genome sequencing’? Name any two organisms whose genome has been sequenced completely.

Q11. What is the advantage of using bacterial artificial chromosomes (BAC) in such sequencing programme?

Q12. Write the procedure of gene cloning.

Q13. What is gene prediction and gene counting?

Q14. Write a short note on ‘proteomics’.

Q15. Write short notes on: SNPs, Functional genomics, Comparative genomics, Expression tag sequence, DNA microarray technology.

Q16. Define the following: Plasmid, Restriction site, transformation, mutation, transfection.

Q17. What are restriction enzymes? Why are they so important in rDNA technology?

Q18. Enlist the various steps involved in rDNA experiment.

Q19. What are the essential features of a vector?

Q20. What does PCR stand for? What are the different steps involved in a PCR reaction?

Q21. What is ‘Insertional Inactivation’?

Q22. What are the disadvantages of using E. coli for production of eukaryotic proteins?

Q23. Differentiate between: a) Blunt ends and sticky ends, b) YAC and BAC, c) Genomic library and cDNA library, d) Microinjection and electroporation.

Q24. Write a short note on RFLP and indicate one of its important applications.

Q25. What is site-directed mutagenesis? What are it’s applications?

Q26. What are the consequences of incorrect folding of a protein?

Q27. Name two human diseases caused by the absence of a protein.

Q28. What is ‘Proteomics’? What are it’s applications?

Q29. Differentiate between protein engineering and genetic engineering.

Questions on Biotechnology and Medicine

Q1. What are monoclonal antibodies? How are they formed?

Q2. What is antenatal diagnosis? What ate it’s applications?

Q3. Write short notes on the following: Gene therapy, genetic counselling, Genetic diseases, Genetic databank, Super bug.

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