Biotechnology Question Bank, Animal Biotechnology, Hybridoma Technology

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Questions on Animal Biotechnology

Q1. What are the requirements for animal cell and tissue culture?

Q2. Write in details a note on animal cell culture medium, it՚s different ingredients and methods of sterilization.

Q3. Discuss in detail different methods of disaggregation of animal tissue.

Q4. What are the advantages and disadvantages of adding serum in the animal culture medium?

Q5. What is the difference between cold trypsinization and warn trypsinization?

Q6. What is immobilized cell culture?

Q7. Write short notes on: Organ culture, Hybridoma technology, tPA, EPO, Somatic cell fusion, MoAB.

Q8. Name two important products from animal cell culture technology. What are their functions?

Q9. Write two important features of animal cell culture. Differentiate between primary and secondary cell cultures.

Q10. What are cell lines? How are the growth characteristics of cell lines determined?

Q11. What is the importance of ph While culturing animal cells? How is the pH maintained in culture media?

Q12. What are some of the characteristics features of normal and transformed cells?

Q13. Discuss the role of serum in culturing animal cells.

Q14. Differentiate between roller bottles and spinner bottles.

Q15. How many haemopoietic cells are supposedly present in mouse? Also indicate how many RBCs, platelets, and B – cells are produced per kilogram body weight?

Q16. How is erythropoietin produced by animal cell culture?

Q17. What is the mode of action of tPA? How is it produced by animal cell culture technology?

Q18. What are the applications of embryonic cell technology?

Q19. Differentiate between monoclonal antibodies and polyclonal antibodies? Write the applications of monoclonal antibodies.

Q20. What are the public concerns regarding the use of genetically engineered products.

Q21. Briefly describe the equipments required for animal cell culture.

Q22. Differentiate between finite cell lines and continuous cell lines.

Q23. Write a brief note on bioethics in animal genetic engineering.

Questions on Biotechnology and Agriculture

Q1. What is artificial insemination? Write in detail about semen storage and sperm sexing in animals.

Q2. What is ‘multiple ovulation’ ? In what ways it can be induced, and embryo transferred in animals?

Q3. What do you mean by embryo splitting and implantation in animals?

Q4. What is embryo cloning?

Q5. What is nuclear transplantation in animals?

Q6. Write a note on cloning of embryonic stem cells.

Q7. What are transgenic animals? Write in brief about transgenic mammals, sheep, and fish.

Q8. What is molecular farming?

Q9. What is the role of molecular markers in livestock improvement?

Q10. Write a short note on ‘Dolly’ .

Q11 Write a note on ‘transgenic breeding strategies and molecular markers’ .

Q12. What is a chimeric mouse?

Q13. Write a brief note on bioethics in plant genetic engineering.

Q14. What are the genetic engineering strategies to create the following traits in

transgenic crops: a) Herbicide tolerance, b) Abiotic stress, c) insect resistance, d) Virus resistance.

Q15. What is the common strategy to produce transgenic crops with delayed ripening and longer shelf life of fruits.

Q16. What is ‘Golden Rice’ ? In what why it is different from the normal rice?

Q17. Write briefly about the benefits of biodegradable plastics that are produced from GM plants.

Q18. What are the advantages of using molecular markers in plant breeding selections over morphological and biochemical markers?

Q19. How can genetically engineered microbes be used for increasing crop productivity and crop protection?

Q20. What biotechnological strategies have helped us to obtain transgenic plants?

Q21. How can transgenic plants be used in the detection and study of DNA sequences regulating gene expression?

Q22. Describe the various techniques utilized for introducing foreign DNA into plant cells.

Q23. What is direct gene transfer?

Q24. Define the following: virus mediated gene transfer, microinjection, liposomes, electroporation, particle gun method, agroinfection