Reverse Transcriptase (RT) : RNA to DNA YouTube Lecture Handouts

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Reverse Transcriptase (RT): RNA to DNA - What Are the Steps & Process? Part of RT-PCR for Covid-19

Title: Reverse Transcriptase

RNA to cDNA

  • Requires reverse transcriptase enzyme, primer and dNTPs
  • Also called 1st strand cDNA synthesis
Reverse Transcriptase
  • Oligo
  • Random
  • Gene specific primer – cDNA yield is lower since only targeting 1 transcript
RNA to cDNA

Reverse Transcriptase Steps

Reverse Transcriptase Steps
  • Enzyme choice is one choice
  • MMLV enzyme is one
  • Superscript III upto 50 degree Celsius – more yield

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